β-Hydroxybutyric acid (β-HB) Content Assay Kit,100T

Brands:Solarbio

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Place of Origin:China

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Shipping:Ice bag

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Shelf Life:6 months under -20℃

Storage:-20℃

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Research Areas:Coenzyme I Series

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β-Hydroxybutyric acid (β-HB), in patients with severe acidosis, NADH production increases due to acidosis, which in turn promotes the ratio of β-hydroxybutyric acid to acetoacetic acid to increase from the normal 2:1 to 16: 1. β-hydroxybutyric acid is of great significance in the diagnosis and treatment of diabetic ketoacidosis. It is also of great significance for the early diagnosis of diabetes. The kit is suitable for serum, plasma, urine and other liquid samples.

Measurement Principle：

At pH8.8 and 37℃, β-hydroxybutyrate reacts under the catalysis of β-hydroxybutyrate dehydrogenase, and NAD+ is oxidized to NADH. In the presence of 1-mPMS, WST-1 can react with NADH to produce water-soluble formazan with a characteristic absorption peak at 450nm. The content of β-HB can be calculated by detecting the wavelength change at 450nm.

Self Provided：

Spectrophotometer/microplate reader, desk centrifuge, pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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Detection Instrument :Spectrophotometer

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