Glutamic-oxalacetic Transaminase(GOT) Activity Assay Kit,50T

Brands:Solarbio

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Place of Origin:China

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Shipping:Ice bag

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Shelf Life:6 months under -4℃

Storage:4℃

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Research Areas:Amino Acid Metabolism Series

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GOT is widely found in animals, plants, microbes and cultured cells. It catalyzes the reversal of amino reactions and is an important enzyme in amino acid metabolism. In addition, GOT is the highest in cardiomyocytes and is commonly used as an assisted examination of myocardial infarction and myocarditis in clinical practice. The serum concentration of liver damage can also be increased.

Measurement Principle：

GOT catalyze α-ketoglutaric acid react with aspartate to produce glutamic acid and oxaloacetic acid. Oxaloacetic acid is further decarboxylated to form pyruvate, pyruvate can react with 2, 4-dinitrophenylhydrazine to produce 2,4-dinitrophenylhydrazone, which shows brownish red in alkaline condition, the activity of GOT enzyme activity can be calculated by measuring the absorbance of 505 nm.

Self Provided：

Spectrophotometer, water bath, desk centrifuge, transferpettor, 1 mL glass cuvette, mortar/homogenizer, ice and distilled water.Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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Detection Instrument :Spectrophotometer/Microplate Reader

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