Synbio Technologies

Artikelnummer: SB-RS-IVT-0005

In vitro Transcription

In vitro transcription takes linear DNA sequences as a template, using T7, T3, or SP6 RNA polymerase to synthesize RNA from the DNA template sequence.

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€9.999,00
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Service Details

Item Details

Service:

Workflow & Deliverables：

Materials Required:

Delivery:

Library QC:

Specification:2 µg (40–9000 nt)

PolyA Tails:• Customized tail
• 100-150A tail (enzymatic method)
• 120A tail
• No tail

Cap:• One stop capping (Clean cap AG, ARCA cap, m7GpppG Cap)
• Enzymatic capping (Cap 0, 1)
• No cap

Modifications:• 5-mUTP
• 5-moUTP
• ψ-UTP
• N1-Me-ψ-UTP
• Cy3, Cy5, AF488
• Unmodified

Purification method:• Silica column purification • LiCl purification • Phenol/chloroform extraction • HPLC

Purity:

length:

Turnaround Time:• IVT: 5-10 BD
• Gene Synthesis: 10-15 BD

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