Lacate Dehydrogenase(LDH) Activity Assay Kit,100T

Brands:Solarbio

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Place of Origin:China

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Shipping:Ice bag

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Shelf Life:6 months under -4℃

Storage:4℃

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Research Areas:Glycolysis Series

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L-Lactate dehydrogenase (L-LDH or LD) is the terminal enzyme of the glycolysis pathway which is found in nearly all living cells (animals, plants, and prokaryotes). L-LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back.

Measurement Principle：

NAD+ and lactic acid are oxidized to pyruvic acid by the catalysis of L-LDH. Pyruvate further reacted with 2,4-dinitrophenylhydrazide to form pyruvate dinitrobenzone, which show brown red color in alkaline solution and the color depth is proportional to the concentration of pyruvate.

Self Provided：

Spectrophotometer/Microplate reader, thermostat water bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice, distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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Detection Instrument :Spectrophotometer

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