Synbio Technologies

Artikelnummer: SB-AD-MAP-0005

Monoclonal Antibody Preparation

Monoclonal antibody technology involves fusing a single B lymphocyte capable of producing antibodies with myeloma cells, resulting in hybrid cells that can generate both antibodies and proliferate indefinitely, thereby enhancing antibody production. This technology has been widely applied in fundamental research, medical diagnosis, and tumor-targeted therapy, among other fields.

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€9.999,00
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Service Details

Item Details

Service:1.Animal Immunization：
Blood collection before immunization
First immunization
(Antigen plus Freund's complete adjuvant)
Second immunization
(Antigen plus Freund's incomplete adjuvant)
Third immunization
(Antigen plus Freund's incomplete adjuvant)
Blood sampling and ELISA test
Fourth Immunization (Antigen is dissolved in PBS or saline)
Animal blood collection
Turnaround Time: 7 ~ 9 weeks

2.Cell fusion and screening：
Cell fusion and screening
Subcloning and cell expansion
Cell line cryopreservation
Turnaround Time: 5 ~ 6 weeks

3.Antibody production and purification：
Hybridoma cell culture
Antibody purification
ELISA, SDS-PAGE, concentration determination
Turnaround Time: 5 ~ 6 weeks

Workflow & Deliverables：

Materials Required:

Delivery:

Library QC:

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PolyA Tails:

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length:

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