Pyrophosphate: Fructose-6-Phosphate-1-Phosphoric Acid Transferase(PFP) Activity Assay Kit,100T

Brands:Solarbio

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Place of Origin:China

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Shipping:Ice bag

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Shelf Life:6 months under -20℃

Storage:-20℃

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Research Areas:Photosynthetic Series

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Pyrophosphate: Fructose-6-phosphate-1-phosphotransferase (PFP, EC2.7.1.90) is a cytosolic enzyme that is widely present in plant tissues and catalyzes the phosphorylation of fructose-6-phosphate like phosphofructokinase. As a result, the single PEP catalytic reaction is a reversible reaction, and pyrophosphate is used instead of ATP, which plays an important role in carbon metabolism of photosynthesis.

Measurement Principle：

PFP catalyzes the conversion of fructose 6-phosphate to fructose 1,6-diphosphate, which is converted to dihydroxyacetone phosphate by the action of aldolase and triose phosphate isomerase, and then catalyzed by α-phosphate glycerol dehydrogenase and NADH to from Glycerol 3-diphosphate and NAD. The change in absorbance at 340 nm reflects the level of PFP activity.

Self Provided：

Low temperture centrifuge, spectrophotometer/microplate reader, water bath/constant temperature incubator, mortar/homogenizer, micro quartz cuvette/96 well plate, transferpettor, ice and distilled water, EP tube.Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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Detection Instrument :Colorimetric

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