Pyruvate Dehydrogenase(PDH) Activity Assay Kit,100T

Brands:Solarbio

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Place of Origin:China

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Shipping:Ice bag

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Shelf Life:1 year under -4℃

Storage:4℃

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Research Areas:Tricarboxylic Acid Cycle Series

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Determination of Significance：

PDH（EC 1.2.4.1） widely exist in animals, plants, microorganism and cultured cells, which is the rate-limiting enzyme of acetylformic acid oxidative and decarboxylate catalyzed by Pyruvate dehydrogenase complex (PDHC). The decarboxylation of acetylformic acid forms hydroxyethyl-TPP, links glycolysis to the three carboxylic acid cycle.

Measurement Principle：

PDH catalyzes the dehydrogenation of acetylformic acid and reduct 2, 6-dichlorophenol indophenol (2,6-DCPIP), which makes the absorption of 605 nm decrease.

Self Provided：

Spectrophotometer/microplate reader, water bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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Detection Instrument :Spectrophotometer

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