Synbio Technologies

Artikelnummer: SB-DS-VL-0007

Variant Libraries

Creating variant libraries is a crucial technique for studying gene function. These libraries are essential for improving protein characteristics, directed evolution of proteins, optimizing antibody sequences, affinity maturation, and target discovery. Researchers can alter specific protein sites or DNA regulatory regions to create a variant library containing tens of thousands of sequences. This approach controls sequence diversity and enhances screening efficiency.

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Service:1.Alanine Scanning Library: Systematic substitution of each residue with alanine exploits the small size and minimal structural impact of alanine to map functionally critical positions.

2.Comprehensive Mutation Coverage: NNK-based single-site mutagenesis covering all 19 alternative amino acids at any position.

3.Site-Saturation Mutagenesis Library: Concurrent saturation of selected continuous or discontinuous sites; accepts all 20 amino acids or customized subsets, with optional skewed codon usage (e.g., Trimer codon mixtures).

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