{"product_id":"human-dna-topoisomerase-1-top1-elisa-kit-96t","title":"Human DNA Topoisomerase 1, TOP1 ELISA Kit, 96T","description":"\u003cp\u003eReleases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL\/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL\/BMAL1 promoter.\u003c\/p\u003e","brand":"BT LAB","offers":[{"title":"Default Title","offer_id":56592536207733,"sku":"BT-E3605Hu-96T","price":370.98,"currency_code":"EUR","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0843\/2671\/1640\/files\/ELISA_Kit_main_1.jpg?v=1766393459","url":"https:\/\/www.labcart.com\/products\/human-dna-topoisomerase-1-top1-elisa-kit-96t","provider":"LabCart Life Science","version":"1.0","type":"link"}