Bacterial Expression

Item Details

Service:Service steps, modular, can place orders in segments
1.Gene synthesis：
• Codon optimization and gene synthesis
• Sub cloning into appropriate expression vector
• Various tags are available, such as His, SUMO,TRX,GST…
• Turnaround Time: ≤2 weeks

2.Protein Expression Testing and Purification：
• Transform plasmids into appropriate bacterial expression strain
• Protein expression evaluation
• Scale up protein expression
• Purification to reach desired protein amounts and purity
• Tag removal and separation of tag-free protein (optional)
• Refolding in case of insoluble protein (optional)
• Turnaround Time: ≤3 week

3.QC & Delivery：
• SDS-PAGE
• Western Blot (optional)
• Bradford assay for quantitation
• Protein delivery
• Turnaround Time: ≤1 week

Workflow & Deliverables：

Materials Required:

Delivery:

Library QC:

Specification:

PolyA Tails:

Cap:

Modifications:

Purification method:

Purity:

length:

Turnaround Time: