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SKU: E-BC-F022_96T-ES
Ferroptosis Inhibitory Protein-1 (FSP-1) Activity Fluorometric Assay Kit, 96T
Ferroptosis inhibitory protein-1(FSP-1) induce non-caspase-dependent apoptosis based on factors such as C-terminal fragment of amino acid series, nuclear translocation and overexpression. Through the FSP1-CoQ10-NAD(P)H pathway and the classical glutathione (GSH)-GPX4 pathway, FSP-1 can protect cells from ferroptosis, and plays a "double-edged sword" role in cell life activities.
The detection principle of this kit: FSP-1 catalyzes the substrate reaction to generate NADH, so that its fluorescence value increases under the excitation wavelength of 535 nm and emission wavelength of 587 nm. Adding inhibitors will inhibit the activity of FSP-1 and cause the rate of increase in fluorescence value to decrease, and the activity of FSP-1 can be calculated by measuring the difference value.
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Product Specification
Basic Information
Brands:Elabscience
Model Number:
Place of Origin:China
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Shipping:Ice bag
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Sensitivity:0.15 U/L
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Shelf Life:12 months
Storage:This product can be stored at -20°C for 12 months with shading light.
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Detection Type:Fluorescence method
Detection Range: 0.15-5.00 U/L
Research Areas:Ferroptosis
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Result Type:Enzyme activity
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Assay Time:1 h
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Detection Instrument :Fluorescence microplate reader
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