Glucoamylase Assay Kit,50T

Brands:Solarbio

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Place of Origin:China

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Shipping:Ice bag

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Shelf Life:6 months under -4℃

Storage:4℃

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Research Areas:Starch Series

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Determination of Significance：

Saccharifying enzyme, glucoamylase (EC3.2.1.3) also known as γ- amylase, which is an extracellular enzyme that is secreted by a series of microorganisms and has exonuclease activity. The main role of Saccharifying enzyme is to hydrolyze α-1,4 glycosidic bonds in sequence from non-reducing ends on the carbon chains such as starch, dextrin, glycogen, etc., and cut off each glucose unit. For amylopectin, when it encounters a branch point, it can also hydrolyze α-1,6 glycosidic bonds, thereby hydrolyzing all amylopectin to glucose. It is one of the important industrial enzyme preparations in China, and is widely used in alcohol, liquor, antibiotics, amino acids, organic acids, glycerol, starch sugar and other industries.

Measurement Principle：

Soluble starch could be hydrolyzed into glucose by saccharifying enzyme. 3, 5-Dinitrosalicylic acid is reduced to brown-red amino compound by co-heating with glucose under alkaline condition. The brown-red amino compound has a maximum absorption peak at 540 nm and the absorbance ratio is in direct proportion to the contents of glucose. The saccharifying enzyme activity is quantified by measuring the color development at 540 nm.

Self Provided：

Spectrophotometer, water bath, balance, centrifuge, adjustable transferpettor, mortar/homogenizer/cell ultrasonic crusher, 1 mL glass cuvette, ice and distilled water.Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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Detection Instrument :Spectrophotometer/Microplate Reader

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