Glutamate（Glu）Content Assay Kit (WST colorimetry),100T

Brands:Solarbio

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Place of Origin:China

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Shipping:Ice bag

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Shelf Life:6 months under -20℃

Storage:-20℃

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Research Areas:Amino Acid Metabolism Series

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Determination of Significance：

Glu is widely present in animals, plants, microorganisms and cultured cells. It is not only one of the 20 amino acids that make up proteins, but also participates in the synthesis of various amino acids through transamination, and is one of the main sources of amino acids in living organisms. In addition, Glu is also the main active ingredient of monosodium glutamate, and is commonly used in food additives and spice production.

Measurement Principle：

Glutamate dehydrogenase (GDH) catalyzes glutamate and NAD to generate α-ketoglutarate, NADH and NH4+. Under the action of 1-mPMS, WST-1 can react with NADH to produce water-soluble formazan, and calculate glutamate acid content.

Self Provided：

Spectrophotometer / microplate reader, centrifuge, transferpettor, water bath / constant temperature incubator, micro glass cuvettes/96 well plates, mortar/homogenizer, sonicator, ice and distilled water.Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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Detection Instrument :Spectrophotometer/Microplate Reader

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