Insect Expression

Item Details

Service:Service steps, modular, can place orders in segments
1.Gene Synthesis (optional)：
• Codon optimization
• Gene synthesis
• Vector construction
• Turnaround Time: Starting from 10 BD

2.Baculovirus preparation：
• Generation of recombinant Bacmid DNA
• Generation of P1 stock, P2 stock and determination of virus titer
• Turnaround Time: 3-5 weeks

3.Validation and analysis of protein expression：
• P2 generation virus infect SF9 cells
• Expression analysis validation (WB)
• Ni column affinity purification
• Turnaround Time: 2-4 weeks

4.Protein Expression and Purification：
• 1 L or larger scale expression
• Turnaround Time: 1-2 weeks

Workflow & Deliverables：

Materials Required:

Delivery:

Library QC:

Specification:

PolyA Tails:

Cap:

Modifications:

Purification method:

Purity:

length:

Turnaround Time: