Synbio Technologies

SKU: SB-AD-PAP-0006

Polyclonal Antibody Preparation

Polyclonal antibodies are a mixture of antibodies that recognize different epitopes of a specific antigen; these antibodies can be produced by collecting antiserums after artificially immunizing the host. Because they can recognize multiple epitopes, polyclonal antibodies have a higher affinity and sensitivity than monoclonal antibodies and are more tolerant to small antigen changes.

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€9.999,00
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Service Details

Item Details

Service:•Antigen Preparation：
Antigen provided by the customer:10 mg of peptide
; 5-8 mg of recombinant protein of more than 85% purity; Small molecular antigens
Synthesized or prepared by LabCart: Design and synthesize polypeptide, coupled with KLH/BSA/OVA
; Codon optimization, gene synthesis, recombinant protein expression and purification
Turnaround Time:2 ~ 4 weeks

•Animal Immunization (mice,rabbits,goat)：
Immunize 3 ~ 4 times;
Turnaround Time: 6 ~ 7 weeks
•Antibody Purification：

Antigen affinity purification or Protein A/G affinity purification;
Turnaround Time: 1 ~ 2 weeks

•Quality Control：
Antibody purity test (SDS-PAGE); specific detection of antigen and antibody (Western Blot analysis); antibody titer detection (ELISA, titer > 1:100000)
Turnaround Time: 1 ~ 2 weeks

•Additional Tests：
Antibody affinity test; antibody labeling; identification of antibody subtypes

Workflow & Deliverables：

Materials Required:

Delivery:

Library QC:

Specification:

PolyA Tails:

Cap:

Modifications:

Purification method:

Purity:

length:

Turnaround Time:

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