Pyruvate Carboxylase(PC) Activity Assay Kit,50T

Brands:Solarbio

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Place of Origin:China

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Shipping:Ice bag

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Shelf Life:6 months under -20℃

Storage:-20℃

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Research Areas:Gluconeogenic Series

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Determination of Significance：

Pyruvate carboxylase (PC, EC 6.4.1.1) is widely present in mitochondria of animals, molds and yeast, but is not found in plants and most bacteria. PC is the main postreaction for oxaloacetate, and is the first rate-limiting enzyme in the gluconeogenesis process.

Measurement Principle：

PC irreversibly catalyzes pyruvate, ATP, CO2 and water to oxaloacetate, ADP and Pi, malic dehydrogenase further catalyzes the formation of malic acid and NAD+ from acetoacetic acid and NADH. The enzyme activity of PC can be reflected by detecting the oxidation rate of NADH at 340 nm.

Self Provided：

Ultraviolet spectrophotometer, desk centrifuge, water bath, adjustable pipette, 1 mL quartz cuvette, mortar/homogenizer, ice and distilled water.Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

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Detection Instrument :Spectrophotometer

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