Yeast Expression

Item Details

Service:Service steps, modular, can place orders in segments
1. Gene Synthesis (optional)：
• Codon optimization
• Gene synthesis
• Vector construction (pPICZα, pPIC9, pGAPZα…)
• Turnaround Time: Starting from 10 BD

2.Electrotransformation and Screening Positive Clones (PCR method)：
• Linearized the plasmid and transformed into pichia pastoris cells to screen positive clones
• Turnaround Time: 2-3 weeks

3.Pilot Protein Expression：
• Three positive clones will be used to verify the expression, then perform expression analysis validation (WB)
• Turnaround Time: 2-3 weeks

4.Protein Expression and Purification：
• 1 L or larger scale expression
• One or more steps purification
• Purity will be detected by SDS-PAGE or Western blot (optional)
• Turnaround Time: 3-4 weeks

Workflow & Deliverables：

Materials Required:

Delivery:

Library QC:

Specification:

PolyA Tails:

Cap:

Modifications:

Purification method:

Purity:

length:

Turnaround Time: